Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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An RNA expression method for aging forensic hair samples.

Author(s): Hampson C, Louhelainen J, McColl S

Publication: J Forensic Sci, 2011, Vol. 56, Page 359-65

PubMed ID: 21281307 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of patient gender and age and storage on the quantification of 18S rRNA and beta actin in hair specimens.

   Summary of Findings:

   18S rRNA and beta actin levels declined by 10% and 40% respectively during the first day. After the first day, beta actin levels continued to decline steadily, but 18S rRNA remained relatively stable until 45 days. Interindividual variability increased with storage. The authors report no bias from patient age or gender, and that quantification of 18s rRNA and beta actin was possible from as little as 1 and 3 hair follicles, respectively.

   Biospecimens

   • Tissue - Hair

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 days 1 day 5 days 15 days 30 days 45 days 60 days 75 days 90 days
   Preaquisition	Patient age	20-25 years 40-45 years
   Preaquisition	Patient gender	Female Male
   Real-time qRT-PCR Specific	Targeted nucleic acid	18S rRNA Beta actin
   Biospecimen Aliquots and Components	Aliquot size/volume	1 follicle 3 follicles 10 follicles

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