Amplification of cDNA via RT-PCR using RNA extracted from postmortem tissues.

Author(s): Phang TW, Shi CY, Chia JN, Ong CN

Publication: J Forensic Sci, 1994, Vol. 39, Page 1275

PubMed ID: 7525856 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to assess whether RNA extracted from postmortem heart and kidney, which resulted from sudden death, could be successfully reverse-transcribed (RT) and amplified via polymerase chain reaction (PCR).

   Summary of Findings:

   The authors report successful extraction of RNA of sufficient quality to allow amplification of the human cardiac beta-myosin heavy chain gene via RT-PCR from both kidney and heart specimens. Ribosomal 28S and 18Sbands were present in all postmortem samples. RNA extracted from kidney specimens, which were stored for a longer duration (5-8 months), showed more degradation than heart specimens (3 months). Nonspecific products were observed after PCR amplification.

   Biospecimens

   • Tissue - Heart
   • Tissue - Kidney

   Preservative Types

   • Frozen

   Diagnoses:

   • Autopsy
   • Not specified

   Platform:

   Analyte	Technology Platform
   RNA	RT-PCR
   RNA	Electrophoresis

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Biospecimen location	Kidney Heart
   Storage	Storage duration	3 months 5 - 8 months
   Storage	Storage temperature	-20 degrees C

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