Nursing blood specimen collection techniques and hemolysis rates in an emergency department: analysis of venipuncture versus intravenous catheter collection techniques.
Author(s): Lowe G, Stike R, Pollack M, Bosley J, O'Brien P, Hake A, Landis G, Billings N, Gordon P, Manzella S, Stover T
Publication: J Emerg Nurs, 2008, Vol. 34, Page 26-32
PubMed ID: 18237663 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of blood collection by IV catheter versus by venipuncture and blood collection site on the rates of hemolysis. Nurses collected 70 specimens using one randomally assigned method and then collected 70 specimens using the other method. All collections via IV catheter were through a newly placed line. A total of 853 specimens (355 venipuncture and 498 IV catheter) were analyzed for hemolysis by blinded laboratory technicians.
Summary of Findings:
A significantly lower rate of hemolysis was found for specimens collected by venipuncture (0.3%) versus those collected via IV catheter (5.6%, p<0.001). Hemolysis rates were not significantly different between IV catheter collection sites. The single hemolyzed specimen was obtained by venipuncture from an anticubital site. The authors also state that catheter size had no significant impact on hemolysis rates.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Morphology Macroscopic observation Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Method of fluid acquisition IV catheter
Venipuncture
Biospecimen Acquisition Cannulation Multiple catheter sizes
Biospecimen Acquisition Anatomical location of blood draw Anticubital vein
Forearm
Hand
Wrist
Unspecified
More than one site