NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

A comparison of IgG anti-rubella activity in frozen serum stored in primary gel separation tubes or secondary tubes.

Author(s): Ellis V, Charlett A, Bendall R

Publication: J Clin Pathol, 2004, Vol. 57, Page 104-6

PubMed ID: 14693850 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of storage container on the measurement of anti-rubella immunoglobulin G (IgG) concentrations in serum after 1 year of frozen storage.

Conclusion of Paper

After 1 year of storage at -20 degrees C, anti-rubella IgG concentrations were not different between the specimens stored in serum separator tubes (SST) and those stored in plastic microtubes.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of storing serum at -20 degrees C for 1 year in primary gel separation tubes as opposed to transferring specimens to new plastic storage tubes on the measurement of anti-rubella IgG concentrations. Specimens were centrifuged and then refrigerated overnight in original collection tubes prior to experimental storage at -20 degrees C, either in same collection tube with new tops, or in a new plastic microtube.

    Summary of Findings:

    After 1 year of storage at -20 degrees C, no differences were found in anti-rubella IgG concentrations between the specimens stored in SST and those stored in plastic microtubes.

    Biospecimens
    Preservative Types
    • Frozen
    Diagnoses:
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Glycoprotein Immunoassay
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Type of storage container Vacutainer Plus SST
    Plastic microtube

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