Reassessment of the rate of fixative diffusion.

Author(s): Start RD, Layton CM, Cross SS, Smith JH

Publication: J Clin Pathol, 1992, Vol. 45, Page 1120-1

PubMed ID: 1479044 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of room temperature (20 degrees C) cold ischemia time on PCNA IHC staining in formaldehyde-fixed specimens.

   Summary of Findings:

   While fixation delays had no significant effect on PCNA grade (low: less than 50% positive nuclei versus high: greater than 50% positive nuclei), fixation delays of 24 h resulted in a 10% average increase in PCNA index. The authors note there was marked intertumoral heterogeneity with respect to PCNA indices for each cold ischemia time.

   Biospecimens

   • Tissue - Breast
   • Tissue - Lung

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Morphology	Light microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Cold ischemia time	30 min 1 h 2 h 4 h 6 h 18 h 24 h

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