Does delay in fixation affect the number of mitotic figures in processed tissue?

Author(s): Cross S S, Start R D, Smith J H

Publication: J Clin Pathol, 1990, Vol. 43, Page 597

PubMed ID: 2199539 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if a fixation delay (30 min - 6 h; defined from the time of arterial clamp placement through storage at 20 degrees C post surgical resection) impacts the number of mitotic figures quantifiable in FFPE tissue.

   Summary of Findings:

   Although there was a great degree of variation between specimens, the authors reported a 30% decrease in mitotic figures with a two-hour delay and a statistically significant 50% decrease with a delay of six hours. They cautioned that the rate at which mitotic figures decrease with increasing fixation delays may be tissue-specific.

   Biospecimens

   • Tissue - Colorectal

   Preservative Types

   • Formalin

   Diagnoses:

   • Normal - Normal Adjacent

   Platform:

   Analyte	Technology Platform
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Unspecified ischemia time	30 min 1 h 2 h 3 h 6 h

   View more