Reproducibility of a semiquantitative measurement of circulating DNA in plasma from neoplastic patients.

Author(s): Frattini M, Balestra D, Verderio P, Gallino G, Leo E, Sozzi G, Pierotti MA, Daidone MG

Publication: J Clin Oncol, 2005, Vol. 23, Page 3163-4; author reply 3164-5

PubMed ID: 15860889 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of frozen storage and within patient variability on the semi-quantitative analysis of DNA content in plasma from patients with colorectal cancer or intestinal polyps. Blood was immediately aliquoted into heparin-coated cryotubes, and plasma was subjected to experimental storage at -20 degrees C. DNA was quantified using the DNA DipStick kit.

   Summary of Findings:

   The authors report semi-quantitative DNA concentrations were concordant between plasma specimens stored for 0 weeks, 2 weeks, 3 months and 3.5 months. Within-patient variability of semi-quantitative cell-free DNA concentrations in 15 patients was low with agreement observed in 85% of the measurements and discrepancies due to single-class shifts.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   DNA	Colorimetric assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	2 weeks 3 months 3.5 months 0 weeks
   Preaquisition	Diagnosis/ patient condition	Colorectal cancer Intestinal Polyps

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