Evaluation of effect of specimen-handling parameters for plasma preparation tubes on viral load measurements obtained by using the Abbott RealTime HIV-1 load assay.

Author(s): Fernandes H, Morosyuk S, Abravaya K, Ramanathan M, Rainen L

Publication: J Clin Microbiol, 2010, Vol. 48, Page 2464-8

PubMed ID: 20484602 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of delayed centrifugation and freezing plasma in situ after centrifugation on HIV load determination

   Summary of Findings:

   No effect of delayed centrifugation was found on HIV load determination. K2 EDTA tubes and plasma preparation tubes performed similarly as did specimens which were aliquoted prior to and after freezing. Variability between measurements was found to be increased in specimens with a viral load below 100 copies/ml, but no effect of specimen handling was found. In conclusion, specimens collected in plasma preparation tubes can be stored for 6 h prior to centrifugation and can be frozen in the same tube without affecting viral load determination.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • AIDS/HIV-related

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-70 degrees C
   Biospecimen Acquisition	Type of collection container/solution	BD Vacutainer plasma preparation tube BD Vacutainer K2 EDTA tube
   Preaquisition	Prognostic factor	Less than 50 viral copies/ml 50-5000 viral copies/ml
   Storage	Time at room temperature	2 h 6 h
   Storage	Type of storage container	Original collection tube New tube
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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