Effect of pneumatic tube delivery system rate and distance on hemolysis of blood specimens.

Author(s): Eviyaoglu O, Toprak G, Tekin A, Basarali M, Kilinç C, Colpan L

Publication: J Clin Lab Anal, 2012, Vol. 26, Page 66-9

PubMed ID: 22467320 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of anticoagulant type, PTS transport, PTS speed and duration, and centrifugation before or after transport on LDH and K levels and markers of blood quality in serum and plasma.

   Summary of Findings:

   K and LDH levels were higher in some plasma and serum specimens when whole blood was transported by PTS rather than hand-delivered (p<0.05), and the magnitude of the effects increased with increasing distance travelled and speed of transport. Changes in LDH and K tended to be larger in plasma that was anticoagulated with citrate than in heparinated plasma, and PTS transport-related increased in K and LDH tended to be higher in plasma than serum, but significance was not evaluated. When blood was centrifuged prior to PTS transport, and serum was transported instead of whole blood, the LDH and K levels were comparable to levels measured in hand-delivered specimens. Moderate to strong correlations between K and LDH levels and distance travelled by PTS were observed in plasma (r=0.628-0.716) and serum (r=0.774-0.871), depending on transport speed. Transport by PTS did not affect cell counts, PT, aPTT, fibrinogen levels or markers of platelet activation.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Cell count/volume	Hematology/ auto analyzer
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Within hospital transportation method	Hand-delivered Pneumatic tube system
   Storage	Specimen transport duration/condition	4.2 m/sec 3.1 m/sec 500 m 1100 m 1380 m 1450 m 2200 m 2900 m Centrifuged Uncentrifuged
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum Whole blood
   Biospecimen Acquisition	Anticoagulant	Citrate Heparin None
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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