A new plastic evacuated tube with plasma separator.

Author(s): Landt M, Wilhite TR, Smith CH

Publication: J Clin Lab Anal, 1995, Vol. 9, Page 101-6

PubMed ID: 7714661 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of tube type, and centrifugation speed (1100 x g or 13600 x g) and duration (1, 2, 3, 5 or 10 min) on platelet removal, lactate dehydrogenase (LDH) levels and plasma recovery.

   Summary of Findings:

   Centrifugation of blood at 13,600 x g reduced platelet counts in the plasma by more than 95% after only 3 minutes and resulted in maximum plasma recovery after just 1 minute. When blood was centrifuged in plastic tubes at 1100 x g, 10 minutes was necessary to effectively remove platelets and recover the maximum amount of plasma. The amount of plasma recovered at each time point was dependent on the hematocrit of the specimen. No effect of centrifugation or tube type was found on LDH.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Coulter counter
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Terumo Venoject II Glass
   Preaquisition	Biomarker level	Hematocrit of 35% Hematocrit of 46% Hematocrit of 58%
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Whole blood
   Biospecimen Aliquots and Components	Centrifugation	Multiple speeds compared Multiple durations compared

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2. Study Purpose

   The purpose of this study was to determine the effects of tube type, partial filling, presence of separator and storage on therapeutic drug concentrations in blood specimens.

   Summary of Findings:

   Concentrations of phenobarbital, carbamazepine, and phenytoin were lower and valproic acid, theophylline, and cyclosporine were higher when blood was collected from pediatric patients in plastic tubes rather than glass tubes; however, only the decrease in carbamazepine was clinically significant. When carbamazepine spiked blood was put in plastic tubes, a 4-5% decrease in carbamazepine was noted, regardless of the presence of a seperator. When carbamazepine spiked blood was stored at 4 degrees C for 24 h, a further decrease in carbamazepine was found, but only when the separator was present and a 1 or 2 mL fill volume was used.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Terumo Venoject II Glass
   Storage	Storage temperature	4 degrees C
   Storage	Storage duration	0 h 24 h
   Storage	Type of storage container	Plastic with separator Plastic without separator
   Biospecimen Aliquots and Components	Aliquot size/volume	1 mL 2 mL 4 mL

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