Haemolysis as an interference factor in clinical chemistry.
Author(s): Sonntag O
Publication: J Clin Chem Clin Biochem, 1986, Vol. 24, Page 127-39
PubMed ID: 3711797 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to ascertain the effects of specimen haemolysis on measurement of clinical chemistry analytes in blood.
Conclusion of Paper
The addition of haemolysed blood to serum resulted in decreased alkaline phosphatase, cholesterol, bilirubin, gamma-glutamyltransferase, albumin (by 1D gel only), and alpha-2-globulin levels, and increased aspartate aminotransferase, alanine aminotransferase, creatinine kinase, acid phosphatase, beta-globin, potassium and lactate dehydrogenase levels.
Studies
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Study Purpose
The purpose of this study was to ascertain the effects of specimen haemolysis on measurement of clinical chemistry analytes in serum specimens. An aliquot of blood was haemolysed by freezing for 20 minutes at -40 degrees C, after which time it was mixed with the remainder of the clotted specimen and centrifuged and filtered to obtain serum without sediment.
Summary of Findings:
The addition of 0.8 g/L hemoglobin to serum resulted in decreased alkaline phosphatase, cholesterol, bilirubin and alpha-2-globulin and increased beta-globin, potassium and lactate dehydrogenase. The addition of 1.7 g/L or more of hemoglobin decreased gamma-glutamyltransferase and increased aspartate aminotransferase and acid phosphatase. Creatinine kinase increased when more than 2.5 g/L of hemoglobin was added and alanine aminotransferase increased. Albumin decreased (by 1D gel only) when more than 3.4 g/L hemoglobin was added. Specimen hemolysis had no clinically significant effects on the measurement of alpha-amylase, calcium, cholinesterase, chloride, cholesterol, creatinine, iron, glutamate dehydrogenase, alpha 1-globulin, gamma-globulin, glucose, uric acid, urea, sodium, phosphate, transferrin or triglycerides.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Lipid Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Protein 1D/2D gels Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Biospecimen components 0.8 g/L hemoglobin
1.7 g/L hemoglobin
2.5 g/L hemoglobin
3.4 g/L hemoglobin
4.2 g/L hemoglobin
5.0 g/L hemoglobin
5.8 g/L hemoglobin
6.6 g/L hemoglobin
Biospecimen Aliquots and Components Hemolysis Freeze/thaw-induced
Hemolysate added
No hemolysate added