The importance of blood sampling site for determination of hemoglobin and biochemistry values in major abdominal and orthopedic surgery.

Author(s): Evron S, Tress V, Ezri T, Szmuk P, Landau O, Hendel D, Schechter P, Medalion B

Publication: J Clin Anesth, 2007, Vol. 19, Page 92-6

PubMed ID: 17379118 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this double-blind study was to determine the effects of location of blood draw and timing of blood collection, with respect to anesthesia and surgery, on levels of hemoglobin, electrolytes, glucose, and lactate, and pH, and blood gas determination. Specimens were obtained from 100 patients undergoing either total hip replacement, revision of total hip replacement, colon resection, or radical cystectomy while under general anesthesia.

   Summary of Findings:

   Lactate levels were significantly higher in peripheral venous blood than in central venous blood or radial arterial blood (p<0.05), and lactate levels were significantly higher in central venous blood than in arterial blood (p<0.05). No other analytes, including partial pressure oxygen, partial pressure carbon dioxide, hemoglobin, glucose, sodium, potassium, and pH, were affected by sampling location. None of the tested analytes were affected by the timing of blood collection, with respect to anesthesia and surgery.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	After induction of anesthesia 1 hour post induction of anesthesia At the end of surgery After 1 hour in recovery room 4 hours after surgery
   Preaquisition	Diagnosis/ patient condition	Undergoing major surgery
   Preaquisition	Anesthesia	Collected after induction of anesthesia Collected 1 hour post induction of anesthesia Collected at the end of surgery Collected after 1 hour in recovery room Collected 4 hours after surgery
   Biospecimen Acquisition	Anatomical location of blood draw	Artery Central vein Peripheral vein

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