Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Unravelling in vitro variables of major importance for the outcome of mass spectrometry-based serum proteomics.

Author(s): West-Nørager M, Kelstrup CD, Schou C, Høgdall EV, Høgdall CK, Heegaard NH

Publication: J Chromatogr B Analyt Technol Biomed Life Sci, 2007, Vol. 847, Page 30-7

PubMed ID: 17112795 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effect of thaw temperature and time on MS profiles from serum.

   Summary of Findings:

   The coefficient of variance (CV) of the MS of serum increased with thaw duration and temperature. The effect of storage time in specimens thawed at 22 degrees C approached the biological variability, but in specimens thawed at 4 degrees C the effect of thaw time remained small when compared to biological variability. Most of the changes in the MS profile were in the low mass range (1200-3000 Da) and were found to represent complement C3f, fibrin alpha, fibrinopeptide A, and kininogen fragments. The 2000 Da region was found to show the most variability between measures.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	MALDI-TOF MS
   Peptide	MALDI-TOF MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Thaw duration	0 min 30 min 60 min 120 min
   Storage	Thaw temperature/condition	0 degrees C 22 degrees C

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2. Study Purpose

   The purpose of this study was to determine the effect of humidity during specimen crystallization on MS from serum.

   Summary of Findings:

   On days when the humidity was extremely low (15%) the authors found it difficult to produce MS results. When the effect of humidity during crystallization on MS plots were examined, humidity was found to explain 73% of variability. Interestingly the humidity did not alter specific peaks, but instead was a more general effect.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	MALDI-TOF MS
   Peptide	MALDI-TOF MS

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage conditions	20% humidity 33% humidity 46% humidity

   View more