The effect of venous occlusion on the level of serum cholesterol.
Author(s): KOERSELMAN HB, LEWIS B, PILKINGTON TR
Publication: J Atheroscler Res, 1961, Vol. 1, Page 85-8
PubMed ID: 13757507 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of venous occlusion on the measurement of cholesterol and sodium in serum as well as plasma volume. Venous occlusion was carried out at a pressure of 80 mm mercury (Hg) for 5 min or 1.5-2 min from one arm while blood was collected from the opposite arm using the same sized needle with no venous occlusion. A No. 1 needle was used during venous occlusion for 5 min while a No. 2 needle was used during the collection with venous occlusion for 1.5-2 min.
Summary of Findings:
Cholesterol levels were an average of 14.8% higher in specimens collected using No. 1 needles after 5 minutes of venous occlusion than with no venous occlusion. Cholesterol levels were an average of 5.3% higher in specimens collected using No. 2 needles during 1.5-2 min of venous occlusion than with no venous occlusion. Both of these increases in cholesterol were higher than the standard error of the laboratory for blind duplicates. The plasma volume was an average of 14.4% lower after the 5 min venous occlusion compared to the specimens drawn without venous occlusion. Serum sodium rose by less than 3% after venous occlusion.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Electrolyte/Metal Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Method of fluid acquisition Tourniquet times compared
Biospecimen Acquisition Needle gauge No. 1
No. 2