Influence of different heparin solutions upon blood gas analysis and biochemical values measured in plasma.

Author(s): Boidin MP, Jorna P

Publication: Intensive Care Med, 1984, Vol. 10, Page 255-60

PubMed ID: 6436351 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of using plasma with sodium heparin (1000 U/mL) or serum on the measurement of clinical chemistry analytes in blood from patients with high levels of hemoglobin.

   Summary of Findings:

   Levels of iPO3/4 and LDH were higher in serum than heparinated plasma while protein levels were lower in serum (p<0.05).

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Gas	Radiometer
   Small molecule	pH
   Small molecule	Clinical chemistry/auto analyzer
   Protein	Colorimetric assay
   Protein	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Flame emission photometry
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Protein	Refractometer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Preaquisition	Diagnosis/ patient condition	Hemoglobin of > 8 mmol/L

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2. Study Purpose

   The purpose of this study was to determine the effects of type, concentration, and volume of heparin anticoagulant added to specimens on clinical chemistry analytes in plasma from patients with high levels of hemoglobin.

   Summary of Findings:

   With increasing volumes of heparin solution there was increased potassium, and decreased total protein, hemoglobin, bicarbonate, base excess and PCO2 for each of three heparin solutions (sodium heparin 5000 U/mL, calcium heparin 25000 U/mL, or sodium heparin 1000 U/mL + electrolytes). For protein and hemoglobin, there was a straight dilution effect. PO2 and O2 saturation were not significantly influenced by the addition of different volumes of any of the three heparin anticoagulants. pH and H+ ion concentration were relatively unaffected by the addition of different volumes of 1000 U/mL sodium heparin, pH decreased and H+ ion concentrations increased when collected in increasing volumes of 5000 U/mL sodium heparin or 25000 U/mL calcium heparin. Interestingly, calcium levels decreased non-significantly when collected in increasing volumes of 5000 U/mL sodium heparin, but increased when collected in increasing volumes of 1000 U/mL sodium heparin (p<0.05). Sodium levels decreased non-significantly with increasing volumes of sodium heparin 1000 U/mL or calcium heparin 25000 U/mL, but increased non-significantly with increasing volumes of 5000 U/mL sodium heparin. With the exception of PCO2, the magnitude of any effects of diluting the heparin was least when sodium heparin 1000 U/mL was used.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Gas	Radiometer
   Gas	Clinical chemistry/auto analyzer
   Protein	Colorimetric assay
   Protein	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Flame emission photometry
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Protein	Refractometer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Hemoglobin of > 8 mmol/L
   Biospecimen Acquisition	Anticoagulant	Sodium heparin Multiple concentrations evaluated Calcium heparin

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