Vitamin C analysis in whole blood, plasma and cells using reduced glutathione as preservative (stabilizer): losses and redistribution.

Author(s): Löwik MR, Schrijver J, Wedel M

Publication: Int J Vitam Nutr Res, 1991, Vol. 61, Page 43-5

PubMed ID: 1856045 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage in the dark on vitamin C levels in whole blood, plasma and packed cells from 27 elderly women. Sodium EDTA whole blood was stabilized with reduced glutathione prior to storage.

   Summary of Findings:

   After 4 h at room temperature, there were insignificant trends toward lower vitamin C concentrations in plasma and higher concentrations of vitamin C in packed cells than when specimens were analyzed within 30 min. Further, there was no significant loss of vitamin C in whole blood specimens. The authors state the data shows a redistribution of vitamin C between plasma and cells, such that plasma was a better medium than cells for the measurement of vitamin C status after storage.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	HPLC

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	<30 min 4 h
   Biospecimen Aliquots and Components	Blood and blood products	Packed cells Plasma Whole blood

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