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Circulating MicroRNA Responses to Postprandial Lipemia with or without Prior Exercise.

Author(s): Springer CB, Sapp RM, Evans WS, Hagberg JM, Prior SJ

Publication: Int J Sports Med, 2021, Vol. , Page

PubMed ID: 34116579 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared the expression of miRNAs in plasma collected prior to and 2 and 4 h after eating a high fat meal from healthy men who had exercised the proceeding day and those who had not. Blood was collected from 10 healthy active men in K₂EDTA blood immediately prior and 2 and 4 h after consuming a high fat meal consisting of heavy whipping cream, sugar, chocolate syrup, and non-fat powdered milk (84% of calories from fat) after a 15 h fast. Blood was collected twice from each participant, once after sedentary behavior for 24-48 h and once after exercising the day prior at 70% maximum oxygen consumption until expenditure of 2.5 MJ. The night prior to the study (15 h), all participants ate a body mass defined quantity of Zone Perfect bars. Plasma was obtained by centrifugation at 1500 x g for 15 min at 4°C and stored at -80°C. miRNA was extracted using the miRNeasy Serum/Plasma Advanced Kit and quantified by real-time RT-PCR.

   Summary of Findings:

   There was a significant interaction between exercise and time after eating a high fat meal on levels of miR-155 (2-way ANOVA P=0.05). ANOVA identified a significant effect of eating on miR-155 (P=0.002), miR126-3p (P=0.02), miR21-5p (P=0.01), miR-146a-5p (P=0.02), miR-181b-5p (P=0.02), and miR-221-3p (P=0.008) levels, but none of the miRNAs were significantly affected by preceding exercise. Levels of miR-155, miR-126-3p, miR181b-5p, and miR-221-3p were 94%, 97%, 79%, and 242% higher, respectively, 2 h after the high fat meal than baseline when the patient had not exercised the day prior (P=0.001, P=0.045, P=0.03, and P=0.045, respectively) but no significant differences were found at 2h after the meal when the patient had exercised the day before. miR-155, miR-126-3p, and miR-21-5p were 88%, 103%, and 62% higher, respectively, 4 h after the high fat meal than baseline when the patient had not exercised the day prior (P=0.01 and P=0.02, respectively) and 110%, 121%, and 75% higher, respectively, when the patient had exercised the day prior to the high fat meal (P=0.0004, P=0.004, and P<0.01; respectively). miR-146a-5p was 184% higher and miR-181-5p was 77% higher 4 h after the high fat meal when the patient had exercised the preceding day (P=0.01 and P=0.01) but not significantly higher when the patient had not exercised.  At 4 h post high fat meal, miR-155 levels were 20% higher in patients who had exercised the day prior than in those that had not (P=0.03), but no other significant differences were found. Levels of miR-92a-3p, miR-150–5p, and miR-222–3p were unaffected by eating a high fat meal or exercise.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient diet	Prior to high fat meal
   Real-time qRT-PCR Specific	Targeted nucleic acid	miR-21 miR-92a miR-126 miR-146a miR-150 miR-155 miR-181b miR-221 miR-222
   Preaquisition	Diagnosis/ patient condition	Sedentary 24-48 h prior Exercised preceding day
   Biospecimen Acquisition	Time of biospecimen collection	0 h after meal 2 h after meal 4 h after meal

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