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Serum and Plasma miRNA Expression Levels in Sudden Sensorineural Hearing Loss.

Author(s): Nunez DA, Abgoon R, Wijesinghe P, Garnis C

Publication: Int J Mol Sci, 2025, Vol. 26, Page

PubMed ID: 39941013 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared levels of eight microRNAs (miRNA, miR) in case-matched serum and plasma collected from men and women. The eight miRNAs were selected due to their implicated role in sudden sensorineural hearing loss. Blood was collected from 8 women and 9 men a median of 3 weeks after sudden sensorineural hearing loss.  Serum and plasma were separated (details not provided) and stored at -80°C. RNA was isolated using the miRNeasy Mini Kit and stored at -80°C. Levels of miR-30a-3p, miR-128-3p, miR 132- 3p, miR-375-5p, miR-590-5p, miR-140-3p, miR-186-5p, and miR-195-5p were quantified by real-time PCR.

   Summary of Findings:

   Levels of all eight of the miRNAs evaluated because of their implicated role in sudden sensorineural hearing loss (miR-30a-3p, miR-128-3p, miR 132- 3p, miR-375-5p, miR-590-5p, miR-140-3p, miR-186-5p, and miR-195-5p) were comparable in serum and plasma specimens and in specimens from men and women. The authors identified 102 miRNA targets of seven of the eight miRNAs evaluated, and enrichment analysis identified enrichment of PI3-Akt, MAPK, RAF, and FOXO pathways.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Preaquisition	Patient gender	Female Male

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