Influence of in vitro hemolysis on hematological testing on Advia 2120.

Author(s): Lippi G, Musa R, Avanzini P, Aloe R, Pipitone S, Sandei F

Publication: Int J Lab Hematol, 2012, Vol. 34, Page 179-84

PubMed ID: 22051137 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of hemolysis on routine hematological testing and plasma lactate dehydrogenase levels. Hemolysis was induced by passing aliquots of blood through a 30-gauge needle 5 (mild hemolysis) or 10 times (gross hemolysis).

   Summary of Findings:

   Mechanically-induced hemolysis (both mild and gross) resulted in significant increases in mean hemoglobin content, mean corpuscular hemoglobin concentration, platelet counts, mean platelet volume, monocyte counts, basophil counts, large unstained cells, and plasma lactate dehydrogenase levels compared to specimens with no hemolysis. Further, the mechanically hemolyzed specimens showed significant decreases in red blood cell counts, hematocrit, mean corpuscular volume, and lymphocyte counts compared to non-hemolyzed specimens. Overall, no significant differences were measured in the white blood cell counts between hemolyzed and non-hemolyzed specimens, but the white blood cell differentials did change as noted above. Hemoglobin, red blood cell distribution width, neutrophil counts, and eosinophils counts were unaffected by mechanically-induced hemolysis.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Cell count/volume	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Whole blood
   Biospecimen Aliquots and Components	Biospecimen components	0-0.5 g/L hemoglobin 4.0-4.5 g/L hemoglobin 9.0-9.5 g/L hemoglobin
   Biospecimen Aliquots and Components	Hemolysis	Fine needle aspiration-induced Not induced

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