Influence of temperature and time before centrifugation of specimens for routine coagulation testing.
Author(s): Salvagno GL, Lippi G, Montagnana M, Franchini M, Poli G, Guidi GC
Publication: Int J Lab Hematol, 2009, Vol. 31, Page 462-7
PubMed ID: 18371059 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of delayed centrifugation on coagulation parameters in trisodium citrate plasma from patients on antivitamin k therapy.
Summary of Findings:
aPTT increased progressively with storage of blood at room temperature or 4 degrees C prior to centrifugation, with significant changes observed after as little as 3 h (p<0.001, and p=0.018, respectively). Compared to specimens centrifuged immediately, PT was decreased after storage of blood for 3 or 6 h at room temperature prior to centrifugation (each p<0.001) and increased after a 24 h delay to centrifugation at 4 degrees C (p<0.001), but after storage of blood for 24 h at room temperature, PT was not significantly different than PT measured without delays to centrifugation. Fibrinogen was not affected by storage of blood at room temperature or 4 degrees C for 3 or 6 h prior to centrifugation, but it was decreased after 24 h at room temperature or 4 degrees C compared to levels measured in specimens processed without delayed centrifugation (p<0.001, both). D-dimer was unaffected by delayed centrifugation.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Glycoprotein Hematology/ auto analyzer Morphology Hematology/ auto analyzer Peptide Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
3 h
6 h
24 h
Storage Storage temperature 2-6 degrees C
Room temperature
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated