UK Biobank Pilot Study: stability of haematological and clinical chemistry analytes.
Author(s): Jackson C, Best N, Elliott P
Publication: Int J Epidemiol, 2008, Vol. 37 Suppl 1, Page i16-22
PubMed ID: 18381388 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to assess the effects of storage on clinical chemistry and hematological analytes in plasma, serum and urine specimens.
Conclusion of Paper
Of the 46 analytes measured, 23 changed by less than 1% per 12 h of storage and another 21 changed between 1 and 3% per 12 h of storage. The largest changes were observed for insulin, which increased by 3.9% per 12 h of storage, and eosinophils, which decreased by 12% per 12 h of storage. However, both changes were highly variable between individuals and not statistically significant. The authors conclude that changes over the first 36 h of storage are small by comparison to individual variability and assay error.
Studies
-
Study Purpose
The purpose of this study was to assess the effects of storage on clinical chemistry and hematological analytes in plasma, serum and urine specimens.
Summary of Findings:
Of the 46 analytes measured, 23 changed by less than 1% per 12 h of storage and another 21 changed between 1 and 3% per 12 h of storage. The largest changes were observed for insulin, which increased by 3.9% per 12 h of storage, and eosinophils, which decreased by 12% per 12 h of storage. However, both changes were highly variable between individuals and not statistically significant. Using a probability model, calcium, cholesterol, fibrinogen and HDL cholesterol all had a probability of a negative bias over time between 0.9 and 0.975. None of the analytes showed a high probability of a positive bias over time. More than 72% of the variability in levels was due to baseline values, and much of the remaining variability was attributable to measurement error. The authors conclude that changes over the first 36 h of storage are small by comparison to individual variability and assay error.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Lipid Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer Cell count/volume Hematology/ auto analyzer Protein Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
12 h
24 h
36 h
Storage Storage temperature -80 degrees C
-196 degrees C