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High-throughput 1H NMR-based metabolic analysis of human serum and urine for large-scale epidemiological studies: validation study.

Author(s): Barton RH, Nicholson JK, Elliott P, Holmes E

Publication: Int J Epidemiol, 2008, Vol. 37 Suppl 1, Page i31-40

PubMed ID: 18381391 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if metabolic profiles of serum specimens are altered by a 24 hour processing delay (at 4 degrees C) prior to freezing, as assessed by NMR analysis.

   Summary of Findings:

   Metabolic profiles of serum were unaffected by storage at 4 degrees C for 24 hours prior to processing and freezing, but displayed evidence of storage duration dependent biochemical alterations after 36 hours at 4 degrees C.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	NMR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 h 24 h 36 h
   Storage	Storage temperature	4 degrees C

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2. Study Purpose

   The purpose of this study was to determine if metabolic profiles of urine specimens are altered by a 24 hour processing delay (at 4 degrees C) prior to freezing, as assessed by NMR analysis.

   Summary of Findings:

   No significant difference in metabolic profile was observed among case-matched urine specimens frozen immediately or stored at 4 degrees for 24 hours prior to freezing.

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	NMR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 h 24 h
   Storage	Storage temperature	4 degrees C

   View more