A GC-TOF-MS study of the stability of serum and urine metabolomes during the UK Biobank sample collection and preparation protocols.
Author(s): Dunn WB, Broadhurst D, Ellis DI, Brown M, Halsall A, O'Hagan S, Spasic I, Tseng A, Kell DB
Publication: Int J Epidemiol, 2008, Vol. 37 Suppl 1, Page i23-30
PubMed ID: 18381390 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The objective of the study was to observe whether the metabolomic composition of serum changes when stored at 4 degrees C for 24 h before being frozen at -80 degrees C, in comparison with being frozen immediately at -80 degrees C after sample preparation.
Summary of Findings:
Serum samples processed immediately and those retained for 24 hour at 4 degrees C before processing and freezing generated similar metabolic profiles, and exhibited less variability than observed between individuals.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule GC-TOF-MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 30 min
24 h
Storage Storage temperature 4 degrees C
-80 degrees C
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Study Purpose
The objective of the study was to observe whether the metabolomic composition of urine changes when stored at 4 degrees C for 24 h before being frozen at -80 degrees C, in comparison with being frozen immediately at -80 degrees C after sample preparation.
Summary of Findings:
Urine samples processed immediately and those retained for 24 hour at 4 degrees C before processing and freezing generated similar metabolic profiles, and exhibited less variability than observed between individuals.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule GC-TOF-MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature 4 degrees C
-80 degrees C
Storage Storage duration 0 h
24 h