Stability of plasma analytes after delayed separation of whole blood: implications for epidemiological studies.
Author(s): Clark S, Youngman LD, Palmer A, Parish S, Peto R, Collins R
Publication: Int J Epidemiol, 2003, Vol. 32, Page 125-30
PubMed ID: 12690023 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to measure the concentrations and determine the stability of albumin, apoA1, apoB, total protein, triglycerides, cholesterol, HDL, LDL, ALT, creatinine, CK, GGT, and AST after delayed separation of whole blood and storage at either 21 or 4 degrees C. Blood was collected into potassium EDTA tubes with aprotinin. All plasma specimens were stored at -80 degrees C once separated and analyzed together in one batch.
Summary of Findings:
Changes in concentration of less than 4% were observed for albumin, apoA1, apoB, total protein, triglycerides, cholesterol, and HDL when whole blood was stored at 21 degrees C or 4 degrees C. LDL concentration changed by less than 7% when stored at either temperature. On the other hand, ALT, creatinine, CK, and GGT concentrations changed upwards of 10% after storing whole blood for 7 days at 21 degrees C. However, these analytes showed less than 4% change after storage at 4 degrees C for up to 7 days. AST was the only analyte measured that showed marked differences in concentration after storage of whole blood at either temperature.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Lipid Clinical chemistry/auto analyzer Lipoprotein Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Steroid Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 d
1 d
2 d
3 d
4 d
7 d
Storage Storage temperature 4 degrees C
21 degrees C
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated