Soluble urokinase plasminogen activator receptor measurements: influence of sample handling.

Author(s): Riisbro R, Christensen IJ, Høgdall C, Brünner N, Høgdall E

Publication: Int J Biol Markers, 2001, Vol. 16, Page 233-9

PubMed ID: 11820717 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature, duration, and freeze-thaw cycling on suPAR concentration in EDTA plasma and serum. Whole blood was subjected to different storage conditions prior to centrifugation. Separated plasma and serum specimens were stored at -80 degrees C prior to analysis.

   Summary of Findings:

   In general, levels of suPAR were higher in serum than in EDTA plasma. The concentrations of suPAR in serum or EDTA plasma were not affected by storage of whole blood at 4 or 20 degrees C for 24 hours prior to centrifugation. However, suPAR concentrations increased significantly in EDTA plasma and in serum when whole blood was stored at 20 degrees C, but not 4 degrees C, for 72 hours prior to centrifugation (p=0.009 and p=0.005, respectively). Freeze-thaw cycling had no effect on EDTA plasma or serum concentrations of suPAR.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	EDTA None
   Storage	Storage temperature	4 degrees C 20 degrees C
   Storage	Storage duration	1 h 3 h 8 h 24 h 72 h
   Storage	Freeze/thaw cycling	1 cycle 2 cycles 3 cycles 4 cycles 5 cycles 6 cycles 7 cycles 8 cycles
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum Whole blood
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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