Neuroendocrine expression in metastatic prostate cancer: evaluation of high throughput tissue microarrays to detect heterogeneous protein expression.
Author(s): Mucci NR, Akdas G, Manely S, Rubin MA
Publication: Hum Pathol, 2000, Vol. 31, Page 406-14
PubMed ID: 10821485 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine if a tissue microarray (TMA) composed of 0.6 mm diameter cores can appropriately capture expression of the heterogeneous neuroendocrine markers CGA and SYN in prostate and metastatic tumors when compared to whole tissue sections. A total of 50 metastatic prostate tumors located in prostate, bone, liver, lung, and adrenal gland were collected from twelve rapid autopsy cases. A single TMA was constructed from 458 cores.
Summary of Findings:
A lower incidence of positive immunostaining for neuroendocrine markers (CGA or SYN) was observed among TMA cores (2.5%) when compared to whole sections (13%); however, it is important to note that these percentages represent the number of slides or cores assessed (100 slides or 218-250 TMA cores) and not patients. While the percentage of neuroendocrine positive cases was reported for whole sections (13%), this information was not provided TMA cores. It is also noteworthy that of the 458 TMA cores placed into the array, only 46% and 55% could be analyzed for SYN and CGA expression, respectively, due to loss or the presence of normal adjacent tissue.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Autopsy
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Protein Tissue microarray Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Type of slide Tissue microarray section
Whole tissue section
Immunohistochemistry Specific Targeted peptide/protein CGA
SYN