The influence of fixation delay on mitotic activity and flow cytometric cell cycle variables.
Author(s): Bergers E, Jannink I, van Diest PI, Cuesta MA, Meyer S, van Mourik JC, Baak JP
Publication: Hum Pathol, 1997, Vol. 28, Page 95-100
PubMed ID: 9013839 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of cold ischemia time on mitotic activity in formaldehyde-fixed lung, breast, and intestinal tissue specimens.
Summary of Findings:
Fixation delays of up to 24 hours at room temperature or 4 degrees C did not affect the average number of mitotic figures. However, tissue quality decreased with time, making mitotic figure counting more difficult.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Neoplastic - Carcinoma
- Normal
Platform:
Analyte Technology Platform Morphology H-and-E microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Cold ischemia time 1 h
2 h
3 h
4 h
6 h
8 h
18 h
24 h
Storage Storage temperature 4 degrees C
Room temperature
-
Study Purpose
The purpose of this study was to determine the effects of cold ischemia time on cell cycle variables in lung, breast, and intestinal tissue specimens that were snap-frozen in liquid nitrogen.
Summary of Findings:
Fixation delays of up to 24 hours at room temperature or 4 degrees C did not affect the average percentage of S-phase cells. However, increases in the percentage of debris and coefficient of variation were seen with room temperature fixation delays of 4 hours or more.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Cell count/volume Flow cytometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Cold ischemia time 1 h
2 h
3 h
4 h
6 h
8 h
18 h
24 h
Storage Storage temperature 4 degrees C
Room temperature