Stability of activated partial thromboplastin time (APTT) test under different storage conditions.

Author(s): Awad MA, Eldeen OA, Ibrahim HA

Publication: Hematology, 2006, Vol. 11, Page 311-5

PubMed ID: 17607579 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage duration and temperature on APTT and FVIII activity in plasma specimens from healthy volunteers and cardiovascular disease patients.

   Summary of Findings:

   For healthy subjects, no significant changes were observed in APTT when plasma was either stored at room temperature or 4 degrees C for up to 6 hours, but increasing APTT was observed with storage at -40 degrees C or when specimens were stored for more than 6 h at room temperature or 4 degrees C. In contrast, for cardiovascular disease patients receiving heparin therapy, APTT test results increased significantly with storage at all temperatures (p<0.05). FVIII activity in healthy patients was significantly lower for all temperatures and storage durations compared to zero time values (p<0.05).

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Cardiovascular Disease

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Other drugs	Unfractionated heparin therapy for 1 day Unfractionated heparin therapy for 2 days
   Storage	Storage temperature	Room temperature 4 degrees C -40 degrees C
   Storage	Storage duration	0 h 6 h 12 h 24 h
   Biospecimen Acquisition	Anticoagulant	Sodium citrate
   Preaquisition	Diagnosis/ patient condition	Healthy Myocardial infarct Unstable angina After heart surgery

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