Specimen processing time and measurement of total insulin-like growth factor-I (IGF-I), free IGF-I, and IGF binding protein-3 (IGFBP-3).

Author(s): Harris TG, Strickler HD, Yu H, Pollak MN, Monrad ES, Travin MI, Xue X, Rohan TE, Kaplan RC

Publication: Growth Horm IGF Res, 2006, Vol. 16, Page 86-92

PubMed ID: 16530441 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine whether serum levels of IGF-I, and IGFBP are affected by delays at room temperature pre- and post-separation.

   Summary of Findings:

   While total IGF-I and IGFBP-3 levels were stable in serum specimens separated by centrifugation prior to storage at room temperature for up to 24 hours, levels in specimens stored for 24 hours prior to separation were significantly elevated. Free IGF-I levels progressively increased with room temperature storage in separated and non-separated specimens.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Diabetes Type 1
   • Normal
   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 h 2 h 4 h 10 h 24 h
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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