Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation.
Author(s): Baechler EC, Batliwalla FM, Karypis G, Gaffney PM, Moser K, Ortmann WA, Espe KJ, Balasubramanian S, Hughes KM, Chan JP, Begovich A, Chang SY, Gregersen PK, Behrens TW
Publication: Genes Immun, 2004, Vol. 5, Page 347-53
PubMed ID: 15175644 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to identify changes in PBMC global gene expression that was a result of a room temperature overnight delay in blood processing.
Summary of Findings:
Microarray analysis revealed that of the 6414 genes assessed in PBMC samples, 2034 displayed a significant alteration in expression as a result of an overnight room temperature delay in blood processing. The average magnitude of change was 6-fold, included over- and under-expression, and affected immediate early genes, early growth response genes, and heat shock proteins, as well as regulators of transcription, cell cycle progression, apoptosis, and immune response.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform RNA DNA microarray Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0 h
Overnight
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Study Purpose
The purpose of this study was to determine whether PBMC or white blood cell gene expression is affected by room temperature delays in blood processing ranging from 2 to 24 hours.
Summary of Findings:
As determined by real-time quantitative RT-PCR, gene expression was affected after a room temperature delay as soon as 2 h after collection. Both up- and down- regulation was observed and trends progressed with delay duration.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform RNA Real-time qRT-PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 0 h
2 h
6 h
12 h
24 h