Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Microsatellite stability in human post-mortem tissues.

Author(s): Hoff-Olsen P, Jacobsen S, Mevåg B, Olaisen B

Publication: Forensic Sci Int, 2001, Vol. 119, Page 273

PubMed ID: 11390139 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine if the length of postmortem interval (PMI) or the occurrence of body decomposition affects the stability of seven somatic microsatellite DNA (STR) markers (tetranucleotides: HUMVWA31/A, HUMTH01, HUMF13A1, HUMFES/FPS; hyperpolymorphic markers HUMAPOAI1, D11S554, HUMACTBP2) in blood and six different tissue types (brain, hair, cartilage, bone, liver, thyroid gland).

   Summary of Findings:

   The authors report that tetranucleotide human microsatellites were stable in blood leucocytes and all of the tissue types examined without evidence of postmortal changes or somatic mutations. This was true for specimens that exhibited no signs of decomposition (median postmortem interval 1 day) as well as specimens obtained from cadavers exhibiting significant decomposition (median postmortem interval 9 days). While profiles were not attained from cartilage, liver, and thyroid for all cases exhibiting decomposition, differences in DNA extraction methods among tissue types may be a confounding variable.

   Biospecimens

   • Tissue - Bone
   • Tissue - Thyroid Gland
   • Tissue - Liver
   • Tissue - Hair
   • Tissue - Cartilage
   • Bodily Fluid - Blood
   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Autopsy

   Platform:

   Analyte	Technology Platform
   DNA	Dot blot or slot blot
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Postmortem interval	1 - 5 days, no decomposition 2 - 132 days, decomposition
   Biospecimen Acquisition	Biospecimen location	Thyroid gland Blood Brain Hair Cartilage Bone Liver

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