Delayed assessment of serum and whole blood estradiol, progesterone, follicle-stimulating hormone, and luteinizing hormone kept at room temperature or refrigerated.

Author(s): Taieb J, Benattar C, Birr AS, Lindenbaum A, Frydman R, Olivennes F

Publication: Fertil Steril, 2000, Vol. 74, Page 1053-4

PubMed ID: 11056261 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of delayed centrifugation and storage of serum on levels of E2, P, LH and FSH in blood from 10 in vitro fertilization patients.

   Summary of Findings:

   P in serum was lower when whole blood was stored at 4 or 25 degrees C for 3 days prior to centrifugation than when serum was analyzed on the collection day, but the difference was clinically irrelevant. The authors state all specimens were obtained during the follicular phase when P was low and further studies will be necessary to determine if P is affected when initial levels are high. No other effects of delayed centrifugation or storage of serum for up to 3 days at 4 or 25 degrees C were observed.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Glycoprotein	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	4 degrees C 25 degrees C
   Storage	Storage duration	0 days 1 day 2 days 3 days
   Biospecimen Aliquots and Components	Blood and blood products	Serum Whole blood
   Preaquisition	Diagnosis/ patient condition	In vitro fertilization patient
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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