Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

Search BRD Papers

Marked gene transcript level alterations occur early during radical prostatectomy.

Author(s): Schlomm T, Näkel E, Lübke A, Buness A, Chun FK, Steuber T, Graefen M, Simon R, Sauter G, Poustka A, Huland H, Erbersdobler A, Sültmann H, Hellwinkel OJ

Publication: Eur Urol, 2008, Vol. 53, Page 333-44

PubMed ID: 17448597 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to assess potential alterations in gene expression induced by prostatectomy, associated warm ischemia time, as well as subsequent cold ischemia durations at room temperature.

   Summary of Findings:

   RNA quality, determined by 28S/18S ratio, was unaffected by the duration of surgery. Of the 91 genes analyzed by low density array (LDA) of pooled samples, 7 increased 2-fold or more within 80 min of blood flow restriction (warm ischemia time), with modest increases induced further by subsequent cold ischemia. Expression of targeted house-keeping genes (GAPDH, HPRT, TFRC) remained stable through out and after surgery. Real-time quantitative RT-PCR (qRT-PCR) analysis in individual samples supported LDA results, with significant increases in EGR1, KRT 17, PIM1, p21, WFDC2, S100P, TNFRSF, and TRIM29 expression after 45-70 min of warm ischemia. Comparisons among biopsies collected during and after surgery with those procured 1 d prior to surgery buttressed the observed changes in gene expression. Statistical analysis revealed that surgical duration (warm ischemia) alone was strongly associated with the observed differences in expression.

   Biospecimens

   • Tissue - Prostate

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma
   • Neoplastic - Normal Adjacent

   Platform:

   Analyte	Technology Platform
   RNA	Real-time qRT-PCR
   RNA	Automated electrophoresis/Bioanalyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient age	50-68 y
   Preaquisition	Biomarker level	3-30 ng/ml PSA
   Preaquisition	Surgery duration	112-171 min
   Preaquisition	Warm ischemia time	0 min 45 min 70 min 80 min
   Biospecimen Acquisition	Cold ischemia time	10 min 20 min 30 min 60 min
   Preaquisition	Blood loss amount	300-1300 ml
   Real-time qRT-PCR Specific	Targeted nucleic acid	GAPDH HPRT TFRC EGR1 KRT17 PIM1 p21 WFDC2 S100P TNFRSF TRIM29
   Biospecimen Acquisition	Organ measurements	25-65 ml Prostate gland volume

   View more