Effects of four different methods of sampling arterial blood and storage time on gas tensions and shunt calculation in the 100% oxygen test.

Author(s): Smeenk FW, Janssen JD, Arends BJ, Harff GA, van den Bosch JA, Schönberger JP, Postmus PE

Publication: Eur Respir J, 1997, Vol. 10, Page 910-3

PubMed ID: 9150334 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of using plastic versus glass syringes, storage temperature, and storage duration on PaO2 and PaCO2 in specimens from patients undergoing a 100% oxygen test. Glass syringes had a 5 mL capacity while plastic syringes had a 3 mL capacity, but as much air as possible was removed from each immediately after specimen collection. Blood gas analysis of specimens collected in glass syringes and stored in ice water for 5 min before analysis (time to transport specimens to the laboratory) was taken as the gold standard.

   Summary of Findings:

   PaO2 was stable for 60 min in blood collected in glass syringes and stored in ice water but decreased significantly after 120 min (p=0.037). Storage of blood in plastic syringes rather than glass and storage of blood in either type of syringe at room temperature rather than in ice water, even for as short as 5 minutes, resulted in significantly lower PaO2 measurements. Increasing storage time lead to further decreases in PaO2 measurements, with PaO2 being the lowest at each time point in blood collected in plastic syringes and stored at room temperature. PaCO2 was not significantly affected by storage of blood in plastic rather than glass syringes, storage at room temperature rather than in ice water, or storage for durations longer than 5 min, with the exceptions of significantly higher values measured in blood from plastic syringes stored at room temperature for 60 or 120 min (p<0.01 and p<0.001, respectively), and in blood from glass syringes stored at room temperature for 120 min (p<0.01). Pulmonary shunt calculations were significantly higher when the gold standard methods were not used.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Undergoing elective aortacoronary artery bypass surgery
   Storage	Storage duration	5 min 15 min 30 min 60 min 120 min
   Storage	Storage temperature	Room temperature In ice water
   Biospecimen Acquisition	Type of collection container/solution	Glass syringe Plastic syringe

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