High-level detection of gene amplification and chromosome aneuploidy in extracted nuclei from paraffin-embedded tissue of human cancer using FISH: a new approach for retrospective studies.

Author(s): Rossi E, Ubiali A, Balzarini P, Cadei M, Alpi F, Grigolatoi PG

Publication: Eur J Histochem, 2005, Vol. 49, Page 53-8

PubMed ID: 15823795 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of performing FISH on whole paraffin sections versus extracted nuclei on the determination of HER2 amplification and ploidy in 43 different carcinoma specimens including breast, ovary, endometrium, mesothelium, and stomach. Nuclei were extracted from a 40 uM thick section identified as wholly neoplastic.

   Summary of Findings:

   A slight increase in the polysomy percentage was observed when CEP17 FISH was performed on extracted nuclei rather than whole sections (p=0.007). 4/43 specimens showed aneuploid signals only on the extracted nuclei slides. While there was no significant difference in the determination of amplification versus no amplification of HER2 between whole sections and extracted nuclei, the numerical HER2/CEP17 ratios were significantly higher when calculated for extracted nuclei compared to whole sections (p=0.015). Nevertheless, discordance in case status was observed in only one case.

   Biospecimens

   • Tissue - Breast
   • Tissue - Ovary
   • Tissue - Endometrium
   • Tissue - Stomach
   • Tissue - Other

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	FISH
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Type of slide	Whole section Extracted nuclei
   FISH Specific	Targeted nucleic acid	Centromere of chromosome 8 Centromere of chromosome 17 HER2

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