Stability of blood gases, electrolytes and haemoglobin in heparinized whole blood samples: influence of the type of syringe.

Author(s): Müller-Plathe O, Heyduck S

Publication: Eur J Clin Chem Clin Biochem, 1992, Vol. 30, Page 349-55

PubMed ID: 1511069 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage and syringe type on blood gas, electrolytes and hemoglobin. 5 commercial polypropylene syringes with different fill volumes, concentrations and types of heparin, and electrolyte balancing were compared to fluid electrolyte balanced 5 mL glass syringes with 10 IU/10^3 L blood of sodium heparin salt.

   Summary of Findings:

   All plastic syringes showed an increase in pO2 after storage for 45 minutes in ice water. The increase was least in hypoxemic specimens or when the Braun Inject (B2) syringes were used. The increase was greatest in plasma compared to whole blood or when Braun Omnifix (B1) syringes were used. The increase was even greater and apparent after 10 minutes instead of 20 min when the blood was stored at room temperature in B1 syringes instead of glass syringes. The pCO2 in specimens in plastic syringes increased slightly with storage in ice water, except when stored in the B1 syringe or when hyperkapnic specimens were used. A slight trend toward decreasing pH after 45 minutes of storage in ice water was noted, but the change was generally below 0.01. Slight changes in sodium, calcium, and potassium were observed, but the amounts were dependent on heparin and syringe type. The changes in hemoglobin were small, but the reproducibility was poor, especially for specimens stored in the B1 syringe.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Small molecule	pH

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Glass syringe Starstedt monvette LH Terumo Preza-pak II Radiometer Art Samp B129 Braun Inject Braun Omnifix
   Storage	Storage temperature	Ice water Room temperature
   Storage	Storage duration	0 min 10 min 20 min 45 min
   Biospecimen Acquisition	Anticoagulant	Lithium heparin Sodium heparin
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Whole blood

   View more