Intraindividual variability of plasma antioxidants, markers of oxidative stress, C-reactive protein, cotinine, and other biomarkers.
Author(s): Block G, Dietrich M, Norkus E, Jensen C, Benowitz NL, Morrow JD, Hudes M, Packer L
Publication: Epidemiology, 2006, Vol. 17, Page 404-12
PubMed ID: 16755268 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of patient age, body mass index (BMI), smoking status and gender on the variability of plasma analytes.
Conclusion of Paper
Most analytes were changed by less than 10% between blood draws. However, alpha carotene varied by 22%, gamma tocopherol varied by 14% and cotinine varied by 24%, but only in those patients exposed to second hand smoke. The number of draws necessary for each analyte was dependent on patient age and smoking status. The required number of blood draws was not dependent on BMI or gender. The authors note that single outliers may have altered the outcome for some analytes.
Studies
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Study Purpose
The purpose of this study was to determine the effects of patient age, BMI, smoking status and gender on the variability of plasma analytes. Two blood draws were obtained 2-4 weeks apart from smokers and non-smokers exposed to second hand smoke.
Summary of Findings:
Most analytes were changed by less than 10% between blood draws. However, alpha carotene varied by 22%, gamma tocopherol varieed by 14% and cotinine varied by 24% but only in those patients exposed to second hand smoke. The number of draws necessary for each analyte was dependent on patient age and smoking status. The required number of blood draws was not dependent on BMI or gender. The authors note that single outliers may have altered the outcome for some analytes.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Steroid Clinical chemistry/auto analyzer Small molecule GC-MS Small molecule Colorimetric assay Small molecule HPLC Lipid Clinical chemistry/auto analyzer Protein Radioimmunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Patient age Less than 45
More than 45
Preaquisition Patient gender Female
Male
Preaquisition Other drugs Smoker
Non-smokers exposed to second hand smoke
Biospecimen Aliquots and Components Aliquot sequential collection 1st collection
2nd collection
Preaquisition Diagnosis/ patient condition Body mass index less than 27.1
Body mass index greater than 27.1