Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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A Physically Active Status Affects the Circulating Profile of Cancer-Associated miRNAs.

Author(s): Faraldi M, Gerosa L, Gomarasca M, Sansoni V, Perego S, Ziemann E, Banfi G, Lombardi G

Publication: Diagnostics (Basel), 2021, Vol. 11, Page

PubMed ID: 33946605 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared the miRNA profiles of plasma from physically active and sedentary men and investigated if the differentially expressed miRNAs overlapped with lists of ccfmiRNAs associated with diagnostic utility for cancer. K₂EDTA blood was obtained from 20 mountain ultra-trail athletes during the 9’th week of training and 10 sedentary men (<150 min/week physical activity), all of whom were healthy (25-40 years old, normal body weight, non-smokers). Sedentary men were asked to refrain from all physical activity for 48 h prior to blood collection while blood was collected from the trained athletes the day before a mountain ultra-trail marathon. Plasma was obtained by centrifugation at 1300 x g for 10 min at room temperature and stored frozen at -80°C. miRNA was isolated from plasma using the miRCURY RNA Isolation Kit and reverse-transcribed using the miRCURY LNA Universal RT miRNA PCR. Levels of 179 miRNAs were assayed using the serum/plasma miRCURY LNA miRNA focus panel. Levels of miRNA were normalized to miR-320d. Hemolysis was evaluated using the ratio of miR-23 to miR-451 and a threshold of >7. Differentially regulated miRNAs in plasma of patients with lung, prostate, colon, liver, and gastroesophageal cancers were identified through literature searches.

   Summary of Findings:

   Thirty-six miRNAs were found to be differentially expressed (≥f2 old difference) in the plasma of active versus sedentary men; 25 of which were up-regulated and 11 down-regulated. Fifteen of the 25 upregulated miRNAs in plasma of athletes and all 11 down-regulated miRNAs were previously reported to be differentially regulated in one or more cancers. These included miRNAs associated with diagnostic utility for lung cancer (13/81), prostate cancer (2/23), colon cancer (12/60), liver cancer (6/53), and gastroesophageal cancer (11/73).

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma
   • Normal

   Platform:

   Analyte	Technology Platform
   RNA	Low density array
   RNA	Real-time qRT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Athlete Sedentary

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