Effect of haemolysis on radioimmunoassay for insulin.
Author(s): Atchison JA, Grizzle WE, Pillion DJ
Publication: Diabetologia, 1988, Vol. 31, Page 403-4
PubMed ID: 3046977 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of hemolysis and assay incubation time on the measurement of insulin in serum by radioimmunoassay (RIA).
Conclusion of Paper
The measured level of insulin increased linearly with increasing hemolysis, but the degree of increase also depended on the incubation time of the RIA.
Studies
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Study Purpose
The purpose of this study was to determine the effects of hemolysis and assay incubation time on the measurement of insulin in serum by RIA. Blood specimens were obtained from 3 volunteers. Hemolysis was induced in the blood specimens by passing the blood through a 22 gauge needle before centrifugation. Serum from hemolyzed blood was added at increasing amounts to non-hemolyzed specimens.
Summary of Findings:
The measured level of insulin increased linearly with increasing hemolysis. A corresponding decrease in the insulin tracer solution was noted with increasing hemolysis. When the incubation time used with the Micromedic RIA was reduced from 16 h to 90 min (incubation time for the Immunophase RIA), the effect of hemolysis on insulin levels was partially attenuated.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein Radioimmunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Hemolysis Fine needle aspiration-induced
Not induced
Radioimmunoassay Specific Incubation time 90 min
16 h