Freezing method affects the concentration and variability of urine proteins and the interpretation of data on microalbuminuria. The Oxford Regional Prospective Study Group.
Author(s): Schultz CJ, Dalton RN, Turner C, Neil HA, Dunger DB
Publication: Diabet Med, 2000, Vol. 17, Page 7-14
PubMed ID: 10691153 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare the stability of urine proteins in case-matched specimens after 6-8 months of storage at -20 or -70 degrees C prior to analysis. Of note, fresh urine specimens were not included as a control.
Summary of Findings:
Compared to urine specimens stored for 6-8 months at -70 degrees C, those specimens stored at -20 degrees C displayed a 30% mean reduction in albumin, a 22% mean reduction in retinol binding protein, and a 17% mean reduction in N-acetyl glucosaminidase. Urinary creatinine was unaffected by the temperature of frozen storage.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Diabetes Type 1
- Normal
Platform:
Analyte Technology Platform Protein ELISA Small molecule Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -20 degrees C
-70 degrees C