Influence of sample type on the interpretation of the oral glucose tolerance test for gestational diabetes mellitus.
Author(s): Neely RD, Kiwanuka JB, Hadden DR
Publication: Diabet Med, 1991, Vol. 8, Page 129-34
PubMed ID: 1827397 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of measuring glucose in capillary versus venous specimens and in whole blood versus plasma during oral glucose tolerance testing.
Summary of Findings:
In general, glucose levels were highest when measured in capillary plasma and lowest in venous whole blood at each time point during oral glucose tolerance testing. Capillary whole blood and venous plasma glucose levels were similar in non-fasting specimens, but in fasting specimens, venous plasma had higher glucose levels than capillary whole blood. In all 4 specimen types, glucose values peaked at the 30 minute time point. Interpretation of results for pregnant women classified 8 women as having IGT when either venous plasma or venous whole blood was analyzed, but only 5 were classified as having IGT when capillary whole blood was analyzed, and 4 were classified as having IGT when capillary plasma was analyzed. The authors recommend the use of venous plasma for oral glucose tolerance testing in pregnant women.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Diabetes Type 2
- Normal
- Pregnant
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Pregnant
Not pregnant
Biospecimen Aliquots and Components Blood and blood products Plasma
Whole blood
Biospecimen Acquisition Time of biospecimen collection After overnight fast
30 min after oral glucose
60 min after oral glucose
90 min after oral glucose
120 min after oral glucose
Biospecimen Acquisition Anatomical location of blood draw Capillary
Vein