Optimizing cord blood sample cryopreservation.
Author(s): Harris DT
Publication: Cytotherapy, 2012, Vol. 14, Page 359-65
PubMed ID: 22175912 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of cryostorage solution and container on the recovery of MNCs from CB. CB was red blood cell (RBC)-depleted, resuspended in autologous CB plasma, or 5% human serum albumin (HSA) at 9 degrees C in cryovials (1.8 mL or 4.5 mL) or cryobags (25 mL), supplemented with DMSO, frozen in a control-rate freezer and stored in liquid nitrogen for at least 2 weeks. Some specimens were wrapped in Nunc plastic sheaths after freezing to prevent cross-contamination during storage in phage contaminated liquid nitrogen. Specimens were thawed at 37 degrees C.
Summary of Findings:
Cell recovery and viability were comparable in specimens frozen in HSA (87% and 92%, respectively) and autologous CB plasma (94% and 92%). respectively). The authors report no effect of diluting autologous CB plasma 1:2 in saline on cell recovery and viability but state that cells stored in 100% autologous CB plasma tended to clump during thawing causing more difficulty in counting and subsequent use. The time it took for specimens in 1.8 mL cryovials, 4.5 mL cryovials, or 25 mL cryobags to thaw at room temperature was similar (39 min, 39 min, and 31 min, respectively). The authors report that plastic sheathing on the outside of the cryovial prevented phage contamination from the liquid nitrogen.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Cell count/volume Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Short-term storage solution Human serum albumin
100% autologous CB plasma
Autologous CB plasma diluted 1:2 in saline
Storage Type of storage container 4.5 mL cryovial
25 mL cryobag
1.8 mL cryovial
Storage Storage conditions Wrapped in plastic sheath
Not wrapped in plastic sheath