Soluble CD40 ligand as a biomarker for storage-related preanalytic variations of human serum.

Author(s): Lengellé J, Panopoulos E, Betsou F

Publication: Cytokine, 2008, Vol. 44, Page 275-82

PubMed ID: 18851919 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to validate CD40 ligand as a biomarker for improper serum specimen handling (extended post-separation delays at varying temperatures, and extensive freeze-thaw cycling).

   Summary of Findings:

   CD40 ligand immunoreactivity was abolished in serum specimens stored at 37 degrees C for 24 hours after separation, or significantly reduced in specimens stored at 16 or 20 degrees C for 24 hours. Multiple linear regression analysis suggested critical exposure thresholds of 12 hours at 37 degrees C and 48 hours at 20 degrees C for serum CD40 ligand stability. Western blot analysis buttressed ELISA findings but provided no evidence of protein degradation. CD40 ligand was stable for up to 10 freeze-thaw cycles.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	ELISA
   Protein	Immunoassay
   Protein	Western blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	16 degrees C 20 degrees C 37 degrees C
   Storage	Storage duration	4 h 12 h 24 h 48 h
   Storage	Freeze/thaw cycling	1 cycle 5 cycles 10 cycles

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