Effects of an intravenous catheter on the local production of cytokines and soluble cytokine receptors in healthy men.

Author(s): Haack M, Reichenberg A, Kraus T, Schuld A, Yirmiya R, Pollmächer T

Publication: Cytokine, 2000, Vol. 12, Page 694-8

PubMed ID: 10843747 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of time of biospecimen collection and obtaining blood through an indwelling catheter rather than a needle on the levels of IL-6, TNF-alpha, and soluble TNF receptors p55 and p75 in plasma. Specimens were obtained through an indwelling catheter over a 10 hour interval (9:00 am-7:00 pm) and also by needle at the last time point (7:00 pm). Plasma was frozen at -20 degrees C until analysis.

   Summary of Findings:

   IL-6 levels increased significantly throughout the day when blood was obtained through an indwelling catheter. TNF-alpha and receptor p75 levels showed small but significant and insignificant linear declines, respectively, throughout the day when specimens were obtained by indwelling catheter, while receptor p55 levels initially decreased but then increased between 3:00 pm and 7:00 pm. Levels of TNF-alpha and the two soluble receptors in specimens collected by indwelling catheter changed by less than 15% throughout the time course analysis, but IL-6 levels doubled. Levels of IL-6 and TNF-alpha were comparable to baseline levels (indwelling catheter, 9:00 am) when a needle was used for collection at 7:00 pm. Levels of p55 were higher in specimens collected with a needle at 7:00 pm than specimens obtained by indwelling catheter at 7:00 pm or 9:00 am. And lastly, levels of p75 were not significantly different between specimens obtained by indwelling catheter at 9:00 am or 7:00 pm (other indwelling catheter collection times were not compared) or those obtained by needle at 7:00 pm.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Indwelling catheter Needle
   Biospecimen Acquisition	Time of biospecimen collection	9:00 am 10:00 am 11:00 am 12:00 pm 1:00 pm 2:00 pm 3:00 pm 6:00 pm 7:00 pm

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