Substantial improvements in performance indicators achieved in a peripheral blood mononuclear cell cryopreservation quality assurance program using single donor samples.
Author(s): Dyer WB, Pett SL, Sullivan JS, Emery S, Cooper DA, Kelleher AD, Lloyd A, Lewin SR
Publication: Clin Vaccine Immunol, 2007, Vol. 14, Page 52-9
PubMed ID: 17050740 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of ambient temperature transportation of blood specimens on yield, viability, and function of cryopreserved peripheral blood mononuclear cells (PBMC).
Conclusion of Paper
No differences were observed in the average PBMC yield or viability between HIV-positive and HIV-negative donors or with exposure to lower ambient temperatures (12 degrees C) during transport of blood specimens when compared to transportation at normal ambient temperatures (22-24 degrees C). Elimination of a 24 hour processing delay through use of a local blood donor significantly increased PBMC viability (p=0.030) but not yield. However, during subsequent experiments, PBMC viability was not significantly impacted by the use of a local blood donor. No significant associations were observed between PBMC viability or yield and cell function.
Studies
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Study Purpose
The purpose of this study was to determine the effects of ambient temperature transportation of blood specimens on yield, viability, and function of cryopreserved PBMC. After transportation at ambient temperatures for 24 hours, blood specimens were processed and PBMC were stored in liquid nitrogen, and then shipped on dry ice for analysis at a central laboratory. Acid-citrate-dextrose was used as an anticoagulant.
Summary of Findings:
No differences were observed in the average PBMC yield or viability between HIV-positive and HIV-negative donors or with exposure to lower ambient temperatures (12 degrees C) during transport of blood specimens when compared to transportation at normal ambient temperatures (22-24 degrees C). Elimination of a 24 hour processing delay through use of a local blood donor significantly increased PBMC viability (p=0.030) but not yield. However, during subsequent experiments, PBMC viability was not significantly impacted by the use of a local blood donor. No significant associations were observed between PBMC viability or yield and cell function; however PBMC clumping was associated with low viability and yield.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- AIDS/HIV-related
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Morphology Light microscopy Protein ELISpot Protein Lymphocyte proliferation assay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
24 h
Storage Storage temperature 22-24 degrees C
12-24 degrees C