Blood collection techniques, heparin and quinidine protein binding.

Author(s): Kessler KM, Leech RC, Spann JF

Publication: Clin Pharmacol Ther, 1979, Vol. 25, Page 204-10

PubMed ID: 759074 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection and storage container, heparin sulfate administration and collection method on the percentage of unbound quinidine in blood from healthy subjects and patients with congestive heart failure, chronic renal failure, or a cardiac catheter. Blood was stored for 24 h at 4 degrees C prior to analysis.

   Summary of Findings:

   Blood collected into or stored in red or green stoppered Vacutainer tubes for 24 h at 4 degrees C had higher concentrations of quinidine then blood collected and stored in all glass systems. There was a similar fraction of unbound quinidine in healthy control subjects and patients with congestive heart failure or chronic renal failure, but the fraction of unbound quinidine increased (p<0.0001) during cardiac catheterization. The elevation of quinidine during cardiac catheterization occurred simultaneously with heparin administration and was found both when the specimen was collected by venipuncture or through the catheter.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Cardiovascular Disease
   • Normal
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Small molecule	Fluorometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Glass syringe Green-stoppered Vacutainer Red-stoppered Vacutainer
   Preaquisition	Diagnosis/ patient condition	Normal volunteer Congestive heart failure Chronic renal failure
   Storage	Type of storage container	Glass-stoppered tube Red-stoppered tube Green-stoppered tube
   Biospecimen Acquisition	Method of fluid acquisition	Cardiac catheter Venipuncture
   Biospecimen Acquisition	Time of biospecimen collection	Prior to heparin At time of heparin injection After cardiac catheterization/heparin injection
   Biospecimen Acquisition	Anatomical location of blood draw	Peripheral vein Heart

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