Spuriously low plasma propranolol concentrations resulting from blood collection methods.

Author(s): Cotham RH, Shand D

Publication: Clin Pharmacol Ther, 1975, Vol. 18, Page 535-8

PubMed ID: 1183138 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood collection container and method on plasma and red blood cell propranolol concentration. Spiked normal blood and patient blood were included in this study.

   Summary of Findings:

   Compared to specimens in glass tubes, spiked blood specimens collected in purple, red or green Vacutainer tubes, but not blue Vacutainer tubes, plastic syringes, or any tested Venoject tubes, had lower plasma propranolol levels. Additionally, purple, red, and green Vacutainer tubes had higher propranolol levels in the red blood cell fraction. When the green stopper used on Vacutainer tubes was replaced with glass or parafilm, the effects of collection container on propranolol levels vanished. A reduction in plasma propranolol levels was observed when specimens were collected by indwelling catheter through polyvinyl tubing rather than by venipuncture.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Red Blood Cells
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Fluorometry
   Small molecule	Equilibrium dialysis

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Glass-stoppered tube Green-stoppered Vacutainer tube Red-stoppered Vacutainer tube Bue-stoppered Vacutainer tube Purple-stoppered Vacutainer tube Red-stoppered Venoject tube Blue-stoppered Venoject tube Green-stoppered Venoject tube Plastic syringe
   Biospecimen Acquisition	Method of fluid acquisition	Venipuncture Indwelling catheter
   Biospecimen Aliquots and Components	Blood and blood products	Red blood cells Plasma Whole blood

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