A Comparison of Bladder Catheterization and Suprapubic Aspiration Methods for Urine Sample Collection From Infants With a Suspected Urinary Tract Infection.
Author(s): Eliacik K, Kanik A, Yavascan O, Alparslan C, Kocyigit C, Aksu N, Bakiler AR
Publication: Clin Pediatr (Phila), 2016, Vol. 55, Page 819-24
PubMed ID: 26423890 PubMed Review Paper? No
Purpose of Paper
This paper compared urine culture results in suprapubic bladder aspiration (SPA) with those in catheter specimens from children under a year with signs of a urinary tract infection.
Conclusion of Paper
Twenty of 83 infants with positive catheter urine specimens had abnormal SPA urinalysis results and 24 SPA were culture positive, but eight of the SPA specimens that were culture positive were negative by urinalysis.
Studies
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Study Purpose
This study compared urine culture results in SPA with those in catheter specimens from children under a year with signs of a urinary tract infection and investigated the sensitivity and specificity of catheter urinalysis for UTI in uncircumcised males. A 21-gauge (G) SPA specimen was obtained within 4 days of the catheter specimen from 83 children (69 male, 24 female) who had not started antibiotics but had >50,000 CFU of a single pathogen per mL in catheterized urine. Urine specimens were analyzed by dipstick and culture. Positive urinalysis was defined as a positive nitrite test, a positive leukocyte esterase test, or a specimen containing ≥10 white blood cells or red blood cells per microliter. A positive catheter culture was defined as ≥50,000 CFU of a single organism/mL, but a positive SPA specimen was defined as the presence of any individual gram-negative bacteria.
Summary of Findings:
Twenty of 83 infants with positive catheter urine specimens had abnormal SPA urinalysis results and 24 SPA were culture positive, but eight of the SPA specimens that were culture positive were negative by urinalysis. When only the 69 male infants (all uncircumcised) were considered, the catheter specimen urinalysis had a sensitivity and specificity of 66.7%, a positive predictive value of 80%, and a negative predictive value of 87.3%.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Other diagnoses
Platform:
Analyte Technology Platform Small molecule Colorimetric assay Protein Colorimetric assay Cell count/volume Microbiological assay Cell count/volume Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Method of fluid acquisition Different urine collection procedures compared
Catheterized urine
Needle