The effect of a pneumatic tube transport system on platelet aggregation using optical aggregometry and the PFA-100.
Author(s): Hübner U, Böckel-Frohnhöfer N, Hummel B, Geisel J
Publication: Clin Lab, 2010, Vol. 56, Page 59-64
PubMed ID: 20380360 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of aspirin therapy and PTS transport of citrated blood on platelet aggregation in PRP from healthy patients. Inside the PTS cartridge, the blood tube was immobilized with foam. Specimens were obtained from 15 subjects before aspirin therapy and then again after 1 week of taking 100 mg aspirin per day.
Summary of Findings:
After PTS transport of whole blood, there was a slight decrease in aggregation amplitudes in PRP from patients before and after aspirin therapy compared to hand-delivered specimens, but this was only significant for ristocetin-stimulated aggregation in untreated subjects (p=0.003) and collagen-stimulated aggregation in aspirin-treated subjects (p=0.0012). Aspirin therapy significantly decreased platelet aggregation in PRP by up to 59% compared to that in PRP collected from subjects prior to treatment. Closure times were increased for collagen/adenosinediphosphate (ADP)-stimulated aggregation of PRP, in untreated subjects, after PTS transport, compared to hand-delivered specimens, and closure times were also increased in blood from subjects on aspirin therapy compared to blood from untreated subjects.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Morphology Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Other drugs No drugs
100 mg aspirin/day for 1 week
Storage Within hospital transportation method Hand-delivered
Pneumatic tube system
Hematology/ auto analyzer Specific Reaction solution Ristocetin
Collagen
Collagen/ADP