Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Split sample analysis of serum folate levels after 18 days in frozen storage.

Author(s): Lawrence JM, Umekubo MA, Chiu V, Petitti DB

Publication: Clin Lab, 2000, Vol. 46, Page 483-6

PubMed ID: 11034534 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of serum for an additional 18 days at -20 degrees C in a frost-free freezer on folate levels. Prior to the start of the 18 days of storage, specimens were collected and transported at room temperature, and aliquoted and frozen at -20 degrees C for 3-11 days.

   Summary of Findings:

   After 18 days of additional storage at -20 degrees C, serum folate values decreased for the vast majority of specimens. An average decrease of 5.0 ug/L (p<0.0001) of serum folate was observed for specimens in the quantifiable range (0.25 ug/L to 39.9 ug/L, after 1:2 dilution, as necessary). For women who were taking vitamin supplements, initial folate levels were consequently higher, and the average decrease in serum folate after 18 days additional storage was 29%. On the other hand, for women who were not taking vitamins and had lower initial serum folate values, the average decrease after 18 days additional storage was only 13% (p<0.002).

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Pregnant

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-20 degrees C Room temperature
   Storage	Storage duration	3-11 d 21-29 d
   Preaquisition	Other drugs	Vitamins containing folic acid No vitamin use

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